Emmanuel Hatzakis

Assistant Professor, Dept. of Food Science and Technology
College of Food, Agricultural, and Environmental Sciences


  • University of Crete, Greece, Chemistry, B.S., 2000
  • University of Crete, Greece, Organic Chemistry, M.S., 2004
  • University of Crete, Greece, NMR Spectroscopy, Ph.D., 2007
  • University of Arizona, USA, NMR Spectroscopy, Postdoctoral Research Associate, 04/21/2008 – 05/10/2010
Professional Experience
  • 08/16/2012 – present: Assistant Professor, Department of Food Science & Technology, Ohio State University.
  • 11/12/2012 – 07-31-2016: NMR Director, Department of Chemistry, Penn State University.
  • 05/11/2010 -12/11/2012: Research Associate Professor/NMR facilities manager, Department of Chemistry & Biochemistry, University of North Carolina at Wilmington.
Research Interests

My research interests include applications of liquid and solid state NMR Spectroscopy in Food Science, Structure Determination and Metabolomics. I am developing analytical tools to determine the authenticity of foods and I will apply novel methodologies to develop agronomic and processing strategies to improve the nutritional value of foods. In addition, I will use a multi-disciplinary research approach that combines spectroscopy, metabolomics, gene expression profiles and microbial analysis to investigate the interaction between nutrition and gut microbiota and how this is related to obesity.

Selected Publications
  • Cai, J.; Zhang, L.; Jones, R.; Correll, J.; Hatzakis, E.; Smith, P.; Gonzalez, F.; Patterson, A. The Anti-Oxidant Drug Tempol Promotes a State of Anti-Obesity Through Functional Metabolic Changes in the Gut Microbiota. Journal of Proteome Research. 2016, 15, 563−571.
  • Dey, K.; Pong,  F.; Breffke, J.; Pavlick, R.; Hatzakis, E.; Pacheco, C.; Sen, A. Dynamic Coupling at the Ångström Scale. Angewandte Chemie. 2016, 55, 1, 113–117.
  • Soo, V.; McAnulty, M.; Tripathi, A.; Zhu, F.; Zhang, L.; Hatzakis, E.; Smith, P.; Agrawal, S.; Nazem-Bokaee, H.; Gopalakrishnan, Salis, H.; S.; Ferry, J.; Maranas, C.; Patterson, A.; Wood, T. Reversing Methanogenesis to Capture Methane for Liquid Biofuel precursors. Microbial Cell Factories. 2016, 15, 11-16.
  • Dais, P.; Misiak, M.; and Hatzakis, E. Analysis of marine dietary supplement using NMR spectroscopy. Analytical Methods. 2015, 7, 5226- 5238.
  • Zhang, L.; Hatzakis, E.; Nichols, R.; Correll, J.; Smith, P.; Perdew, G.; Patterson,A. Metabolic responses of mice to dietary TCDF revealed by a combination of global and targeted analysis. Environmental Science and Technology. 2015, 49, 8067–8077.
  • Zhang, L.; Correll, R.; Tanaka, N.; Murray, I.; Smith, P.; Hao, R.; Albert, I.; Hatzakis, E.; Gonzalez, F.; Perdew, G.;. Patterson, A. Aryl Hydrocarbon Receptor Activation by Persistent Organic Pollutants Impacts Gut Microbiome-Host Metabolic Homeostasis. Environmental Health Perspectives. 2015, 123, 679-688. Science Selections, p. A187.
  • Dais, P, Hatzakis, E. Quality assessment and authentication of virgin olive oil by NMR spectroscopy. A critical review. Analytica Chimica Acta. 2013, 90, 39-51.
  • Agrawal P.; Hatzakis, E; Guo, K.; Yang, D. Solution Structure of the Biologically Relevant G- Quadruplex Element in the Human VEGF Promoter. Nucleic Acids Research. 2013. 41, 10584–10592.
  • Hatzakis, E; Okamoto, K.; Yang, D.Thermodynamic stability and folding kinetics of the major G-quadruplex and its loop-isomers formed in the Nuclease Hypersensitive Element in the human c-Myc promoter-Effect of loops and flanking segments on the stability of parallel-stranded intramolecular G-quadruplexes. Biochemistry. 2010, 49, 9152–9160.
  • Orfanakis, A.; Hatzakis, E.; Pergantis, S.; Rizos, A.; Dais P. Structure elucidation of Polyglycerol Polyricinolate (PGPR) by light scattering, LC-MS and NMR spectroscopy.J. Amer. Oil Chem. Soc. 2012, 90, 39-51.
  • Hatzakis, E.; Dagounakis, G.; Agiomirgianaki, A.; Dais, P. A Facile NMR Method for the Quantification of Total Free and Esterified Sterols in Virgin Olive Oil. Food Chemistry, 122, 346-352.
  • Hatzakis, E.; Koidis, A; Boskou, D.; Dais, P. Determination of phospholipids in olive oil by 31P NMR Spectroscopy. J. Agric. Food Chem. 2008, 56, 6232-6240.
  • Dais, P.; Spyros, A.; Christoforidou, S.; Hatzakis, E.; Fragaki, G.; Agiomirgianaki, A.; Salivaras, E.; Siragakis, G.; Daskalaki, D.; Margari, M.; Brenes, M.Comparison of Analytical Methodologies Based on 1H and 31P NMR Spectroscopy with Conventional Methods of Analysis for the Determination of Some Olive Oil Constituents. J. Agric. Food Chem. 2007, 55, 577-584.
  • Hatzakis, E.; Archavlis, L.; Dais, P. Determination of Glycerol in Wines Using 31P-NMR Spectroscopy. J. Amer. Oil Chem. Soc. 2007, 84, 615-619.
  • Hatzakis, E.; Dais, P. Determination of Water Content in Olive oil by 31P NMR Spectroscopy. J. Agric. Food Chem.2008, 56, 1866-187.
  • Hatzakis, E.; Dais, P. Detection and quantification of free glycerol in virgin olive oil by 31P NMR Spectroscopy. J. Amer. Oil Chem. Soc. 2009, 87, 29-34.
  •  Mathé, E.; Patterson, A.; Haznadar, M.; Manna, S.; Krausz, K.; Bowman, E.; Shields, P.; Idle, J.; Smith, P.; Anami, K.; Kazandjian, D.; Hatzakis, E.; Gonzalez, F.; Harris, C. Non-invasive urinary metabolomic profiling identifies diagnostic and prognostic markers in lung cancer. Cancer Research. 2014, 74, 3259-3270.
  • Aumiller, W.; Davis, B.; Hatzakis, E.; Keating, C. Interactions of Macromolecular Crowding Agents and Cosolutes with Small Molecule Substrates: Effect on Horseradish Peroxidase Activity with Two Different Substrates. Journal of Physical Chemistry B. 2014. 118, 10624−10632.
  • Mathad, R.; Hatzakis, E.; Dai, J.; Yang, D. c-Myc promoter G-quadruplex formed at the 5’-end of NHE III1 element: insights into biological relevance and parallel-stranded G-quadruplex stability. Nucleic Acids Research. 2011, 39, 9023-9033. 
  • Chen, Y; Agrawal, P; Brown, R; Hatzakis, E; Hurley, L; Yang,D. The Major G-Quadruplex Formed in the Human Platelet-Derived Growth Factor Receptor β (PDGFR-β) Promoter Adopts a Novel Broken-Strand Structure in KSolution. J. Amer. Chem. Soc. 2012, 134, 13220−13223.